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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Synthesis of acridone derivatives via heterologous expression of a plant type III polyketide synthase in Escherichia coli

Fig. 2

Screening of PKS and coenzyme ligase for the in vivo synthesis of DHA and NMA. a Authentic 2,3-dihydroxyquinoline (DHQ); b authentic anthranilate; c authenic N-methylanthranilate; d products from E. coli harboring pC-CmACS-BadA; e products from E. coli harboring pC-RgACS-BadA; f products from E. coli harboring pC-CmACS-pqsA; g products from E. coli harboring pC-RgACS-pqsA. E. coli harboring pC-CmACS-BadA (d), pC-RgACS-BadA (e), pC-CmACS-pqsA (f) or pC-RgACS-pqsA (g) were supplied with 100 μM anthranilate (d, e) or N-methylanthranilate (f, g). P1 and P3 were DHQ. P2 and P4 were determined to be DHA by NMR. S was unreacted N-methylanthranilate. P5 and P6 were determined to be NMA by NMR. U was unidentified product which seemed to be an intermediate of DHA and its retention time was slightly different from that of NMA

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Microbial Cell Factories

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