Fig. 3

PHA production by the P. putida mutants and cell growth of the mutants. a Effects of deletion of the GIs on the PHA yield and CDW of the mutants. b Determination of the key genomic fragments leading to the improvement in the PHA yield and CDW by stepwise deletion of the five genomic fragments between the mutant KTU-U7 and KTU-U13. c Effects of deletion of the glucose dehydrogenase gene gcd on the PHA yield and CDW of the mutant KTU-U13. To accumulate PHA, the mutants were incubated in M9 minimal medium supplemented with 20 g/l glucose for 60 h at 30 °C and 180 rpm on a shaker. The data are mean values ± standard deviations from three replicates