Fig. 2
From: Identification and engineering of 32 membered antifungal macrolactone notonesomycins
a Biosynthetic gene cluster arrangement for notonesomycins A and B. b Polyketide synthase assembly line within the notonesomycin BGC. KS, AT, ACP, ER, KR, DH and TE refers to ketosynthase, acyltransferase, acyl carrier protein, enoylreductase, ketoreductase, dehydratase and thioesterase respectively. c Schematic of CRISPR–Cas9 mediated knock-out (KO) of nbc20 and nbc21. Shown is a UV chromatogram (210 nm) of extracts from the wild type Streptomyces sp. A793 (WT) with the intact notonesomycin gene cluster and the nbc20, 21 double KO mutant (counts). Arrow indicates accumulated notonesomycins A (1) and B (2)