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Table 3 Colony area (cm2) of T. atroviride, T. reesei, and T. virens grown in VMM supplemented with different concentrations of phosphite (Phi; 0.25, 0.5, 0.75, 1, and 2 mM)

From: Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter

 T. atrovirideT. reeseiT. virens
Conidia
 PDA58.1 ± 0.2***60.1 ± 0.558.1 ± 0.4
 No P28.5 ± 0.1*****29.6 ± 0.3*****36.9 ± 0.8*****
 Pi49.0 ± 0.1***43.7 ± 0.5*****50.5 ± 0.5
 0.25 Phi17.3 ± 0.5*****1.6 ± 0.4*****32.1 ± 0.8****
 0.5 Phi5.1 ± 0.2*****1.3 ± 0.2*****11.3 ± 0.4*****
 0.75 Phi2.0 ± 0.1*****1.1 ± 0.04*****4.6 ± 0.3*****
 1 Phi1.1 ± 0.07*****1.0 ± 0.004*****1.2 ± 0.02*****
 2 Phi0.9 ± 0.0*****1.0 ± 0.04*****1.0 ± 0.03*****
Mycelium plugs
 PDA53.7 ± 0.5*****59.8 ± 0.353.8 ± 0.6*****
 No P38.3 ± 0.3*****24.1 ± 0.5*****44.8 ± 0.6*****
 Pi55.9 ± 0.9*****42.1 ± *****51.6 ± 0.5
 0.25 Phi34.7 ± 0.6**31.4 ± 0.4*****38.6 ± 0.2*****
 0.5 Phi20.0 ± 0.8*****29.9 ± 0.6*****31.2 ± 0.2*****
 0.75 Phi17.7 ± 0.5*****23.6 ± 0.4*****22.3 ± 0.3**
 1 Phi11.8 ± 0.6*****22.3 ± 0.4*****18.4 ± 0.3*****
 2 Phi4.2 ± 0.2*****15.1 ± 0.2*****12.1 ± 0.1*****
  1. Vogel’s minimal media (VMM) with phosphate (Pi) and without phosphorus (P) source (No P), and potato dextrose agar (PDA) used as control treatments. Conidia and mycelium plugs were used as inoculum. The values are indicated as the mean value ± SE (n = 3)
  2. Colony area included the spot where the inoculum was placed
  3. ** Significant at p < 0.01, *** significant at p < 0.001, ***** significant at p < 0.00001; ANOVA-Tukey HSD