Skip to main content

Table 2 Colony area (cm2) of T. atroviride grown in VMM media supplemented with different concentrations of phosphite (Phi; 1, 2, 3, 4, and 5 mM)

From: Assessment of the ptxD gene as a growth and selective marker in Trichoderma atroviride using Pccg6, a novel constitutive promoter

 

PDA

No P

Pi

Phi

1

2

3

4

5

Conidia

60.6 ± 0.36

36.7 ± 0.39*****

50 ± 0.12****

0.6 ± 0.12

0.7 ± 0.05

0.7 ± 0.11

0.7 ± 0.08

0.6 ± 0.12

Mycelium plugs

59.9 ± 0.05

52.8 ± 0.56*****

58.4 ± 0.46

12.2 ± 0.12*****

8.3 ± 0.24*****

7.0 ± 0.48*****

5.9 ± 0.92*****

2.9 ± 0.55*****

  1. Vogel’s minimal media (VMM) with phosphate (Pi) and without phosphorus (P) source (No P), and PDA used as control treatments. Conidia and mycelium plugs were used as inoculum. The values are indicated as the mean value ± SE (n = 3)
  2. Colony area included the spot where the inoculum was placed
  3. ***** Significant at p < 0.00001; ANOVA-Tukey HSD
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us