Fig. 5

Characterization and growth of T. atroviride transgenic strains in media supplemented with phosphite. a Growth of six Trichoderma transformants (ccg6_OPT-3, -5, -6 and pki1_OPT-1, -4, -6) harboring ccg6_OPT and pki1_OPT constructs and the Trichoderma wild type strain (TaWT) in modified liquid Vogel’s minimal media supplemented with 1 mM phosphite (Phi) as the phosphorus (P) source. Media without P (No P) and with phosphate (Pi) as the P source were used as controls. Cultures were photographed 7 days after the inoculation. b Dry weight (mg mL⁻¹) of the mycelia produced by TaWT and transgenic strains in (a). Data are expressed as mean ± SE, n = 3 (p < 0.05). Different letters indicate statistical difference. c Relative expression of the ptxD gene in Trichoderma transgenic strains ccg6_OPT-3, -4, -5, and -6, and pki1_OPT-1, -2, -3, -4, -5, -6 determined by quantitative real time PCR (qRT-PCR). T. atroviride IMI 206040 (TaWT) strain and gpd (glyceraldehyde-3-phosphate dehydrogenase) gene were used as negative and internal controls, respectively. d PTXD enzymatic activity expressed as Absolute Fluorometric Units (A.F.U.) determined in T. atroviride transgenic strains ccg6_OPT-3, -5, and -6, and pki1_OPT-1, -3, and -6, and TaWT as negative control