Fig. 1

Transcriptional activity of ccg6 gene in Trichoderma atroviride. a Transcriptomic profile of ccg6, gdp and Knox1 genes in Trichoderma atroviride IMI 206040 (TaWT) according RNA-seq data sets publicly available at the European Nucleotide Archive (https://www.ebi.ac.uk/ena/) and organized into five groups: 1, 2, 3, 4 and 5 (see Materials and Methods section). Expression data was gene level-summarized [30], and estimated counts were normalized using the GeTMM (Gene length corrected Trimmed Mean of M-values) method [31]. b qRT-PCR analysis of the ccg6 gene in T. atroviride grown under different conditions including carbon (-C) and nitrogen starvation (-N), and using different carbon sources (2% of glucose, sucrose or mannitol). Culture in potato dextrose broth (PDB) media was used as control treatment. c qRT-PCR analysis of the ccg6 gene in T. atroviride grown in Volgel’s minimal media (VMM) and PDB at 2, 4 and 6 days after inoculation. The gpd and elF-4 genes were used as internal controls. Data are expressed as mean ± SE, n = 3 (p < 0.05). Different letters indicate statistical difference