Table 2 Plasmids used in this study
From: Improving l-serine formation by Escherichia coli by reduced uptake of produced l-serine
Plasmid | Relevant characteristics | Source |
|---|---|---|
pKD13 | amp and kan markers | [24] |
pKD46 | amp markers, temperature-sensitive | [24] |
pCP20 | amp and Chl markers, temperature-sensitive | [24] |
pMD-19 | PCR cloning vector | Takara |
pSC | Low copy number, kan marker, p15A replicon, lambda PR and PL promoters | Stock in lab |
pSC-08 | pSC derivative, carrying serAfr under PL promoter, serB under PR promoter, serC under PR promoter and pgk under PL promoter | Stock in lab |
pSC-11 | pSC derivative, carrying sdaC under PR promoter | This work |
pSC-12 | pSC derivative, carrying cycA under PR promoter | This work |
pSC-13 | pSC derivative, carrying sstT under PR promoter | This work |
pSC-14 | pSC derivative, carrying tdcC under PR promoter | This work |