Table 1 Plasmid, strain used in this study
Plasmids, strains | Characteristics | Origin/AbR | Source |
|---|---|---|---|
Plasmid | |||
 pACYCDuet-1 | PT7::MSC | p15A/CmR | Novagen |
 pH3BN | Ptac::gadB-SH3D, gadC-SH3L | ColE1/AmpR | [41] |
 pGB100 | PT7::dr1558 | p15A/CmR | This study |
 pGB101 | PT7::gdhA-TT7 | p15A/CmR | This study |
 pGB102 | PT7::icdA, gdhA-TT7 | p15A/CmR | This study |
 pGB103 | PT7::dr1558-PT7::icdA, gdhA-TT7 | p15A/CmR | This study |
 pREDET | PBAD::gam, beta, alpha, recA | pSC101/AmpR | Gene Bridges |
 pMloxC | lox66-Cm-lox71 cassette | Co1E1/AmpR, CmR | [54] |
 pJW168 | Cre-recombinase | pSC101/AmpR | [55] |
Strain | |||
 BL21(DE3) | fhuA2 [lon] ompT gal (λ DE3)[dcm] ∆hsdS |  | NEB |
 GB200 | BL21(DE3) ΔgabT |  | This study |
 GB300 | BL21(DE3) ΔgabT, ΔsucA |  | This study |
 WGB100 | BL21(DE3)/pGB100, pH3BN |  | This study |
 WGB200 | GB200/pGB100, pH3BN |  | This study |
 DGB101 | BL21(DE3)/pGB101, pH3BN |  | This study |
 DGB102 | BL21(DE3)/pGB102, pH3BN |  | This study |
 DGB201 | GB200/pGB101, pH3BN |  | This study |
 DGB202 | GB200/pGB102, pH3BN |  | This study |
 DGB203 | GB200/pGB103, pH3BN |  | This study |
 DGB301 | GB300/pGB101, pH3BN |  | This study |
 DGB302 | GB300/pGB102, pH3BN |  | This study |
 DGB303 | GB300/pGB103, pH3BN |  | This study |