Fig. 6
From: Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli
The RPGPs-assisted CRISPR/Cas9 system for the 100-kb fragment deletion in E. coli. a Diagrams of p-PBAD-Cas9 and RPGPs pDG-R-X series for genomic deletion. p-PBAD-Cas9 plasmid was used to express Cas9 protein induced by L-arabinose. pDG-R-X was used to express paired gRNAs without inducer. b Deletion of 100-kb genomic fragment in E. coli. c Representative PCR results of a 100-kb fragment deletion. Colonies were randomly picked for PCR screening, and a wild-type colony served as control