Table 2 sequence of the primers used in this study
Name | Sequence (5′–3′) | Experiment |
|---|---|---|
rnpB forward | TCGTGAGGATAGTGCCACAG | Quantitative RT-PCR analysis |
rnpB reverse | GGAAGTTTCTTCCCCAGTCC | |
flv3B RT forward | TTTGGTGGAAGATGTGCTGC | |
flv3B RT reverse | GCCAATGTAAGTTAGGCGCA | |
patB forward | AGGGGCGATGTAAAGTGGAA | |
patB reverse | TTGACTGCTCGACTGTAGCA | |
hoxE forward | GCGTCACCAGTATCAGCAAG | |
hoxE reverse | TGGGGCGCTAGGGAAAATAA | |
hoxF forward | ACCCGGCTGAATCTGGTTTA | |
hoxF reverse | AAGCCTGTGTTGCGGATTTT | |
hoxH forward | CTGGACAGGTAAACGATGCG | |
hoxH reverse | ACAAATCCGCGCTGTAATCC | |
hoxY forward | TTTCCTTTGGTGACTGTGCG | |
hoxY reverse | GGTTGATATCGGCTGCTTGG | |
ppatB forward | TATAAGATCTGTCTTTAAATATACATGGTTTGGG | Cloning of patB promoter |
ppatB reverse | TATAGAATTCGAGCTCGTCGACCCGGGATCCATCGATGGGCCCCATATAACTTTCTTCCCACCC | |
flv3B forward | TAT CCCGGG ATG GTA TCG ATG TCT ACG ACC | |
flv3B reverse | TAT AGTACT TTA GTA ATA GTT GCC TAC TTT GCG | |
Strp forward | AATTCCCCTGCTCGCGCAGG | Construction of the hupL deleted mutant |
Strp reverse | AGCTTAGTAAAGCCCTCGCT | |
all0678 forward | TTCGATATCTAGATCTCGAGTCAATTAATGACTTTTGACTAATTA | |
all0678 reverse | AGTAGACGGAGTATACTAGTGCAACTTTCGGAGCG | |
Strp-all0678 forward | CCTGCGCGAGCAGGGGAATTCATATAACTGCTGTGGCA | |
Strp-all0678 reverse | AGCGAGGGCTTTACTAAGCTGTTTAAACGCAGAGGGG |