Fig. 3
H2 production kinetics. a Wild type or WT/patB-fvl3B were grown in nitrate-containing medium until OD 750 nmm = 0.8. Heterocyte formation was induced by transferring the strains to a nitrate-free medium during 24 h. The strains were then incubated under light intensities of either 20 µE/m2 or 60 µE/m2, and H2 production was assessed by chromatography as explained in the methods section during 4 days. The values represent Mean ± SEM (n = 8). b Wild type, WT/patB-fvl3B, ∆hupL or ∆hupL/patB-fvl3B strains were grown under light intensities of 60 µE/m2. Hetrocyte formation and H2-production were respectively induced and performed as described above. The values represent Mean ± SEM (n = 8)