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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Yeast display platform technology to prepare oral vaccine against lethal H7N9 virus challenge in mice

Fig. 1

Construction of yeast display vector and analysis of the expression of HA protein. a Schematic diagram of S.cerevisiae EBY100/pYD5-HA. The HA-Aga2 fusion protein binds to Aga1 through two disulfide bonds after its secretion from the S.cerevisiae. Aga1 is the first subunit of the yeast a-agglutinin receptor. A GS linker is inserted between Aga2 and HA to stabilize the fusion protein expression. b Western blotting analysis. Lane 1: HA glycoprotein. Lane 2: Deglycosylation of HA protein. Lane 3: S.cerevisiae EBY100/pYD5 control. Lane 4: Deglycosylation of S.cerevisiae EBY100/pYD5. c Immunofluorescence microscope. S.cerevisiae EBY100/pYD5 (left) and S.cerevisiae EBY100/pYD5-HA (right) (magnification 400×). d Flow cytometric analysis. S.cerevisiae EBY100/pYD5 (left) and S.cerevisiae EBY100/pYD5-HA (right). e Quantification of S.cerevisiae EBY100/pYD5-HA expressing HA protein by indirect ELISA. The values were obtained from three independent experiments. Bar = mean ± SD

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