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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Relative contributions of non-essential Sec pathway components and cell envelope-associated proteases to high-level enzyme secretion by Bacillus subtilis

Fig. 3

Secretion of AmyE, AmyL or BPN’ by strains lacking individual cell envelope-associated proteases. AmyE-, AmyL- or BPN’-producing strains lacking the sppA, tepA, rasP, prsW, wprA, yqeZ, htrA or htrB genes, as well as the respective wild-type (wt) control, were grown for 16 h in MBU medium at 37 °C. Next, cells and growth media were separated by centrifugation, and proteins in the growth medium fractions were analyzed by LDS-PAGE and SimplyBlue SafeStaining as described for Fig. 1. *, the effects of a rasP deletion were previously described [26]

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