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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

Fig. 6

Influence of additional lacO sites on cellular LacI concentrations. Proteins of ~ 1.2 × 107 cells were separated with SDS-PAGE and analyzed on western blots, probed with an anti-LacI antibody. a Western blot of BL21 wild-type cells and B<2lacO-A1> cells, which were grown without IPTG, 0.01 mM IPTG, or 0.5 mM IPTG. (M) PageRuler™ Plus Prestained Protein Ladder. b Fold changes of band intensities determined in panel a are shown relative to the levels observed in 0 mM IPTG BL21-wt cells. Error bars indicate the standard error of the mean (n = 3)

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