Fig. 4From: Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systemsFlow cytometry analysis of single-cell expression of GFPmut3.1. Induction was performed with 0 (gray, not induced), 0.005 (red), 0.01 (blue), or 0.5 mM (green) IPTG. A–D: T5 promoter controlled by: a three lacO, b two lacO, c one lacO, or d one lacO/lacIQ sequences. e–g A1 promoter controlled by: e two lacO, f one lacO, or g one lacO/lacIQ sequences. h The T7 expression system is used as a referenceBack to article page