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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

Fig. 4

Flow cytometry analysis of single-cell expression of GFPmut3.1. Induction was performed with 0 (gray, not induced), 0.005 (red), 0.01 (blue), or 0.5 mM (green) IPTG. A–D: T5 promoter controlled by: a three lacO, b two lacO, c one lacO, or d one lacO/lacIQ sequences. eg A1 promoter controlled by: e two lacO, f one lacO, or g one lacO/lacIQ sequences. h The T7 expression system is used as a reference

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