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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Escherichia coli σ70 promoters allow expression rate control at the cellular level in genome-integrated expression systems

Fig. 3

Influence of lac operators on expression rate control, shown by the change in on-line GFPmut3.1 fluorescence in fed-batch-like microtiter cultivations. The dashed vertical lines indicate the time of induction. Induction was performed with 0 (gray, not induced), 0.005 (red), 0.01 (blue), or 0.5 mM (green) IPTG. A–D: The T5 promoter is controlled by: a three lacO, b two lacO, c one lacO, and d one lacO/lacIQ sequences. e–g The A1 promoter is controlled by e two lacO, f one lacO, and g one lacO/lacIQ sequences. h The T7 expression system is used as a reference. The Y-axis scale is adjusted to the respective expression rates. The mean relative GFP fluorescence intensity (rfu) represents triplicate samples

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Microbial Cell Factories

ISSN: 1475-2859

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