Strain or plasmid | Relevant characteristics | Source or references |
---|---|---|
E. coli | ||
DH5α | F– endA1 Φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 relA1 hsdR17(rK–mK +) deoR supE44 thi-1 gyrA96 phoA λ–, strain used for cloning | [57] |
S17-1 | ΔrecA, endA1, hsdR17, supE44, thi-1, tra + , strain used for conjugation of G. oxydans | [62] |
G. oxydans | ||
IK003.1 | G. oxydans 621H Δupp ΔgdhS::sdhCDABE Δpdc::ndh ΔgdhM::sucCD | [17] |
IK003.1-igr1::fdhSCL | IK003.1 with the fdhSCL genes of G. japonicus under the control of the P264 promoter integrated between GOX0013 and GOX0014 (GOX_RS01200 and GOX_RS01205) | This work |
IK003.1-igr2::fdhSCL | IK003.1 with the fdhSCL genes of of G. japonicus under the control of the P264 promoter integrated between GOX0028 and GOX0029 (GOX_RS01280 and GOX_RS01285) | This work |
IK003.1-igr3::fdhSCL | IK003.1 with the fdhSCL genes of G. japonicus under the control of the P264 promoter integrated between GOX0038 and GOX0039 (GOX_RS01330 and GOX_RS01335) | This work |
IK003.1 Δsdh::fdhSCL | IK003.1 with the fdhSCL genes of G. japonicus under control of the P264 promoter replacing the genes GOX2095-6 (GOX_RS11750) encoding an inactive sorbitol dehydrogenase (authentic genomic frameshift) | This work |
IK003.1::fdhSCL2 | IK003.1-igr2::fdhSCL with an additional copy of the fdhSCL genes of G. japonicus under the control of the P264 promoter integrated between GOX0038 and GOX0039 | This work |
Plasmids | ||
pBBR1p264-fdhSCL-ST | pBBR1MCS-2 derivative containing the promoter region of GOX0264 upstream of the fdhSCL genes of G. japonicus NBRC3260 with a C-terminal Strep-tag II-encoding sequence fused to the 3′-end of fdhL | [30] |
pAJ63a | KmR, FUS, derivative of pk18mobGII, integration vector for Δupp based counter-selection | [35] |
pAJ63a-igr1::fdhSCL | KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter between GOX0013 and GOX0014 (GOX_RS01200 and GOX_RS01205) | This work |
pAJ63a-igr2::fdhSCL | KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter between GOX0028 and GOX0029 (GOX_RS01280 and GOX_RS01285) | This work |
pAJ63a-igr3::fdhSCL | KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter between GOX0038 and GOX0039 (GOX_RS01330 and GOX_RS01335) | This work |
pAJ63a Δsdh::fdhSCL | KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter with simultaneous deletion of GOX2095-6 (GOX_RS11750) | This work |