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Table 1 Strains and plasmids used in this study

From: Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose

Strain or plasmid Relevant characteristics Source or references
E. coli
 DH5α F– endA1 Φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 relA1 hsdR17(rK–mK +) deoR supE44 thi-1 gyrA96 phoA λ–, strain used for cloning [57]
 S17-1 ΔrecA, endA1, hsdR17, supE44, thi-1, tra + , strain used for conjugation of G. oxydans [62]
G. oxydans
 IK003.1 G. oxydans 621H Δupp ΔgdhS::sdhCDABE Δpdc::ndh ΔgdhM::sucCD [17]
 IK003.1-igr1::fdhSCL IK003.1 with the fdhSCL genes of G. japonicus under the control of the P264 promoter integrated between GOX0013 and GOX0014 (GOX_RS01200 and GOX_RS01205) This work
 IK003.1-igr2::fdhSCL IK003.1 with the fdhSCL genes of of G. japonicus under the control of the P264 promoter integrated between GOX0028 and GOX0029 (GOX_RS01280 and GOX_RS01285) This work
 IK003.1-igr3::fdhSCL IK003.1 with the fdhSCL genes of G. japonicus under the control of the P264 promoter integrated between GOX0038 and GOX0039 (GOX_RS01330 and GOX_RS01335) This work
 IK003.1 Δsdh::fdhSCL IK003.1 with the fdhSCL genes of G. japonicus under control of the P264 promoter replacing the genes GOX2095-6 (GOX_RS11750) encoding an inactive sorbitol dehydrogenase (authentic genomic frameshift) This work
 IK003.1::fdhSCL2 IK003.1-igr2::fdhSCL with an additional copy of the fdhSCL genes of G. japonicus under the control of the P264 promoter integrated between GOX0038 and GOX0039 This work
Plasmids
 pBBR1p264-fdhSCL-ST pBBR1MCS-2 derivative containing the promoter region of GOX0264 upstream of the fdhSCL genes of G. japonicus NBRC3260 with a C-terminal Strep-tag II-encoding sequence fused to the 3′-end of fdhL [30]
 pAJ63a KmR, FUS, derivative of pk18mobGII, integration vector for Δupp based counter-selection [35]
 pAJ63a-igr1::fdhSCL KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter between GOX0013 and GOX0014 (GOX_RS01200 and GOX_RS01205) This work
 pAJ63a-igr2::fdhSCL KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter between GOX0028 and GOX0029 (GOX_RS01280 and GOX_RS01285) This work
 pAJ63a-igr3::fdhSCL KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter between GOX0038 and GOX0039 (GOX_RS01330 and GOX_RS01335) This work
 pAJ63a Δsdh::fdhSCL KmR, FUS, derivative of pAJ63a for integration of the fdhSCL genes under the control of the P264 promoter with simultaneous deletion of GOX2095-6 (GOX_RS11750) This work