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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose

Fig. 4

Cultivation of the indicated G. oxydans strains in a RAMOS device with 80 g/L fructose and 150 mM MES. Depicted is a the residual fructose concentration and the 5-KF concentration (determined by HPLC method B), the yield g/g, and the optical density (OD600) after 29 h, b the oxygen transfer rate (OTR), c the carbon dioxide transfer rate (CTR), and d the respiratory quotient (RQ, shown for OTR values above 5 mmol/L/h). The strains were cultivated in complex medium with 80 g/L fructose and 150 mM MES at 30 °C, 350 rpm, VL = 10 mL in 250 mL flasks, pHstart = 6 and a shaking diameter of 50 mm. Shown are mean values of duplicates and the values determined for optical density, the concentrations of fructose and 5-KF, and the resulting yields in the two experiments are shown as stars

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