Fig. 3
Cultivation of G. oxydans IK003.1-igr1::fdhSCL in a RAMOS device with 80 g/L fructose and different MES buffer concentrations. Depicted is a the residual fructose concentration (red), the 5-KF concentration (green), the yield g/g (grey), and the pH (blue), b the oxygen transfer rate (OTR) and c the total oxygen consumption (TOC). Cultivations were performed at 30 °C, 350 rpm, VL = 10 mL in 250 mL flasks, pHstart = 6 and a shaking diameter of 50 mm in complex medium with 80 g/L fructose and MES buffer at concentrations of 0 mM, 50 mM, 100 mM and 150 mM. Shown are mean values of duplicates and the concentrations determined for fructose and 5-KF in the two experiments as well as the resulting yields are shown as stars