Fig. 4

IgG subclasses analysis. The phenotype of the induced humoral immune response. Anti-TTFC IgG1 (a), IgG2b (b), IgG2c (c), and IgG3 (d) levels detected in mice serum on days 14, 21, and 35. Not-immunized (naïve and probiotic) groups did not produce anti-TTFC antibodies and were not reported in the figure. The data represent the mean (± standard error) of reciprocal endpoint titers. Equal letters mean no statistical difference (p > 0.05) and different letters mean a statistical difference (p < 0.05) between the experimental groups