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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709

Fig. 5

Optimization of plasmid-mediated expression and analysis effect analysis of different strains. a The confirmation of the expression vectors by agarose gel electrophoresis. a-1, confirmation of pWHA by digestion of BamH I/Sph I with about 1500 bp and 8000 bp, a-2, Confirmation of pLYA by digestion of Sac I/Kpn I with about 1500 bp and 6000 bp; b Investigation of the aprE expression level of different strains. The left Y axis indicated AprE enzyme activity and the right Y axis indicated aprE transcriptional level

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Microbial Cell Factories

ISSN: 1475-2859

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