Fig. 4From: Optimized expression and enhanced production of alkaline protease by genetically modified Bacillus licheniformis 2709Confirmation of the aprE disruption and assaying alkaline protease activity of the mutant. a Verification of the single-crossover recombinant with a band of 1250 bp; b Verification of the double-crossover mutant with a band of 1100 bp; c Alkaline protease activity assay in Buttermilk plate of the aprE mutantBack to article page