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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Fig. 8

Purification of pGH protein after fermentation in optimized-pGH–Ssa1/Sis1 (pGH1–Ssa1/Sis1) of Pichia pastori X33. a Intracellular soluble pGH protein in broken cells supernatant, lane 1, F fraction, lane 2, W1 fraction, lane 3, W2 fraction, lane 4, E1 fraction, lane 5, E2 fraction, lane M, Dual Color standard molecular weight pretreated Marker, lane 6, E3 fraction. b The insoluble pGH protein in broken cells sedimentation. Lane 1, F fraction, lane 2, W1 fraction, lane 3, W2 fraction, lane 4, E1 fraction, lane 5, E2 fraction, lane 6, E3 fraction, lane 7, E4 fraction, lane M, the standard molecular weight Marker of 3452 wide type protein

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Microbial Cell Factories

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