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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Fig. 6

Detection of co-expression of pGH with Bip–PDI in Pichia pastoris.a SDS–PAGE analysis of soluble and insoluble protein fractions from Pichia pastoris X33 expressing optimized pGH alone and with Bip–PDI co-expression. Lane 1, Pichia pastoris X33 of control sample, lane M, the standard molecular weight Marker of 3452 wide type protein, lanes 2 and 3, X33-pPICZA-optimized pGH (pGH1) and X33-pPICZA-optimized pGH–Bip–PDI (pGH1–Bip/PDI) for the expression of soluble pGH protein in broken cells supernatant for 12 h, lanes 4 and 5, pGH1 and pGH1–Bip/PDI for the expression of insoluble pGH protein in broken cells sedimentation for 12 h, lanes 6 and 7, pGH1 and pGH1-Bip/PDI for the expression of soluble pGH protein in broken cells supernatant for 24 h, lanes 8 and 9, pGH1 and pGH1–Bip/PDI for the expression of insoluble pGH protein in broken cells sedimentation for 24 h. b Western-blot analysis of soluble and insoluble protein fractions from Pichia pastoris X33 expressing optimized pGH alone and with Bip–PDI co-expression. Lane 1, Pichia pastoris X33 of control sample, lanes 2 and 3, X33-pPICZA-optimized pGH (pGH1) and X33-pPICZA-optimized pGH–Bip–PDI (pGH1–Bip/PDI) for the expression of total pGH protein in whole cells cultivation for 12 h, lane M, Dual Color standard molecular weight pre dye Marker, lanes 4 and 5, pGH1 and pGH1–Bip/PDI for the expression of soluble pGH protein in broken cells supernatant cultivation for 12 h, lanes 6 and 7, pGH1 and pGH1–Bip/PDI for the expression of soluble pGH protein in broken cells supernatant cultivation for 24 h

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Microbial Cell Factories

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