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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall breaking improved stability of porcine growth hormone

Fig. 2

Expression of pGH in Pichia pastoris X33. a Intracellular expression of pGH protein by SDS–PAGE. Lane 1, Pichia pastoris X33 of control sample, lane 2, the expression of pGH protein in the whole cells after cultivation for 12 h, lane 3, the expression of pGH protein in the broken cells supernatant after cultivation for 12 h, lane 4, the expression of pGH protein in the whole cells after cultivation for 24 h, lane 5, the expression of pGH protein in the broken cells supernatant after cultivation for 24 h, lane M, the standard molecular weight Marker of 3452 wide type protein. b Intracellular expression of pGH protein by Western-blot. Lane M, Dual Color standard molecular weight pre-dye Marker, lane 1, the expression of pGH protein in the broken cells supernatant after cultivation for 12 h, lane 2, the expression of pGH protein in the whole cells after cultivation for 12 h, lane 3, the expression of pGH protein in the broken cells supernatant after cultivation for 24 h, lane 4, the expression of pGH protein in the whole cells after cultivation for 24 h, lane 5, Pichia pastoris X33 of control sample

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Microbial Cell Factories

ISSN: 1475-2859

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