New 6,19-oxidoandrostan derivatives obtained by biotransformation in environmental filamentous fungi cultures

Table 1 Products’ accumulation during the conversions of the substrate (1)

Microorganism	Compounds found in the reaction mixture	Retention time by GC (min)	Biotransformation time (days)
Microorganism	Compounds found in the reaction mixture	Retention time by GC (min)	1	3	6	10
Mucor hiemalis KCh W2	2	3.94	67	36	11	7
	3	4.07	21	56	73	68
	1	4.61	11	1	–	–
Penicillium commune KCh W7	2	3.94	9	1	–	–
	1	4.61	91	2	–	–
	4	6.47	–	97	100	100
Penicillium chrysogenum KCh S4	2	3.94	18	–	–	–
	1	4.61	82	1	–
	4	6.47	–	99	100	100
Beauveria bassiana KCh BBT	2	3.94	40	78	–	–
	1	4.61	45	–	–	–
	4	6.47	15	12	84	81
	5	8.44	–	–	9	9
Beauveria bassiana KCh J1	2	3.94	7	14	15	21
Beauveria bassiana KCh J1	1	4.61	93	86	85	79
Isaria fumosorosea KCh J2	2	3.94	22	29	52	64
Isaria fumosorosea KCh J2	1	4.61	78	71	48	36
Beauveria caledonica KCh J3.3	2	3.94	18	31	31	31
Beauveria caledonica KCh J3.3	1	4.61	75	64	65	65
Beauveria caledonica KCh J3.4	2	3.94	94	100	100	100
Beauveria caledonica KCh J3.4	1	4.61	6	–	–	–
Fusarium acuminatum KCh S1	2	3.94	72	90	89	87
Fusarium acuminatum KCh S1	1	4.61	25	5	2	2
Trichoderma atroviride KCh TRW	2	3.94	72	93	92	94
Trichoderma atroviride KCh TRW	1	4.61	24	2	–	–
Isaria farinosa KCh KW1.1	2	3.94	3	0	–	–
	1	4.61	0	–	–	–
	6	6.59	42	26	9	2
	7	5.11	53	59	65	58
	8	8.72	0	1	6	9

Biotransformation conditions: 100 mL of cultivation medium (3% glucose, 1% aminobac) in 300 mL Erlenmeyer flasks, 25 °C, 150 r/min for 10-day transformation. Data are the average of 3 independent experiments. Standard errors were in the range: 0–5

ISSN: 1475-2859