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Table 2 List of E. coli MG1655(DE3) strains used in fermentation experiments

From: Heterologous caffeic acid biosynthesis in Escherichia coli is affected by choice of tyrosine ammonia lyase and redox partners for bacterial Cytochrome P450

Identifier

Plasmid name

Enzymes expressed

Source

s01

IR64

6His-CYP199A2 F185L N∆7

This study

IR54

PdR, Pux

c22

6His-RgTAL

s02

IR64

6His-CYP199A2 F185L N∆7

This study

IR54

PdR, Pux

c71

6His-FjTAL

s03

IR64

6His-CYP199A2 F185L N∆7

This study

IR54

PdR, Pux

c72

SeSam8

s04

IR64

6His-CYP199A2 F185L N∆7

This study

c50

6His-Pux, PdR

s05

IR64

6His-CYP199A2 F185L N∆7

This study

c62

6His-Pux, PuR

s06

IR64

6His-CYP199A2 F185L N∆7

This study

c86

6His-Pdx, PdR

s07

c25

PCNA3-CYP199A2 F185L N∆7

This study

c28

6His-PCNA2-Pux, PCNA1-PdR

s08

c25

PCNA3-CYP199A2 F185L N∆7

This study

c63

6His-PCNA2-Pux_PCNA1-PuR

s09

c25

PCNA3-CYP199A2 F185L N∆7

This study

c88

6His-PCNA2-Pdx, PCNA1-PdR

s10

c96

PCNA1-GGS-CYP199A2 F185L N∆7

This study

c97

6His-PCNA2-Pux, PCNA3-(GGGS)2-PdR

s11

c96

PCNA1-GGS-CYP199A2 F185L N∆7

This study

c98

6His-PCNA2-Pux, PCNA3-(GGGS)2-PuR

s12

c96

PCNA1-GGS-CYP199A2 F185L N∆7

This study

c106

6His-PCNA2-Pdx, PCNA3-GGS-PdR

s13

IR64

6His-CYP199A2 F185L N∆7

This study

c50

6His-Pux, PdR

c22

6His-RgTAL

s14

c25

PCNA3-CYP199A2 F185L N∆7

This study

c28

6His-PCNA2-Pux, PCNA1-PdR

c71

6His-FjTAL

s15

IR64

CYP199A2 F185L N∆7

This study

c62

6His-Pux, PuR

c71

6His-FjTAL

s16

c25

PCNA3-CYP199A2 F185L N∆7

This study

c63

6His-PCNA2-Pux, PCNA1-PuR

c71

6His-FjTAL

s17

c96

PCNA1-GGS-CYP199A2 F185L N∆7

This study

c106

6His-PCNA2-Pdx, PCNA3-GGS-PdR

c71

6His-FjTAL

s18

c84

6His-Pux, 6His-CYP199A2F185L N∆7

This study

c62

6His-Pux, PuR

c71

6His-FjTAL

s19

IR64

6His-CYP199A2 F185L N∆7

This study

c62

6His-Pux, PuR

c185

6His-FjTAL, Pux

  1. 6His, N-terminal hexahistidine tag; GGS, glycine–glycine-serine linker; (GGGS)2, tandem repeat of glycine–glycine-glycine-serine linker