Table 1 List of plasmids used in caffeic acid production strains
Plasmid name | Backbone | Enzyme encoded in MCSI | Enzyme encoded in MCSII | Source |
|---|---|---|---|---|
IR54 | pKVS45 | PdR-Pux operon | n.a. | [18] |
IR64 | pCDFduet | / | 6His-CYP199A2 F185L N∆7 | [18] |
c22 | pRSFduet | 6His-RgTAL | / | This study |
c25 | pCDFduet | / | PCNA3-CYP199A2 F185L N∆7 | This study |
c28 | pETduet | 6His-PCNA2-Pux | PCNA1-PdR (opt) | This study |
c50 | pETduet | 6His-Pux | PdR (opt) | This study |
c62 | pETduet | 6His-Pux | PuR | This study |
c63 | pETduet | 6His-PCNA2-Pux | PCNA1-(GGGS)2-PuR | This study |
c71 | pRSFduet | 6His-FjTAL | / | This study |
c72 | pRSFduet | 6His-SeSam8 | / | This study |
c84 | pCDFduet | 6His-Pux | 6His-CYP199A2F185L N∆7 | This study |
c86 | pETduet | 6His-Pdx | PdR (opt) | This study |
c88 | pETduet | 6His-PCNA2-Pdx | PCNA1-(GGGS)2-PdR(opt) | This study |
c96 | pCDFduet | / | PCNA1-GGS-CYP199A2 F185L N∆7 | This study |
c97 | pETduet | 6His-PCNA2-Pux | PCNA3-(GGGS)2-PdR (opt) | This study |
c98 | pETduet | 6His-PCNA2-Pux | PCNA3-(GGGS)2-PuR | This study |
c106 | pETduet | 6His-PCNA2-Pdx | PCNA3-GGS-PdR (opt) | This study |
c185 | pRSFduet | 6His-FjTAL | Pux | This study |
Plasmid backbones | ||||
 Backbone | Antibiotic resistance | Copy number | Origin of replication | |
 pKVS45 | carbenicillin | ~ 10 | p15A | |
 pCDFduet | spectinomycin | 20–40 | CloDF13 | |
 pRSFduet | kanamycin | > 100 | RSF1030 | |
 pETduet | carbenicillin | ~ 40 | ColE1 | |