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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Effect of the TetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona

Fig. 3

Growth curve and glucose consumption of S. pogona, S. pogona-Δsp1418 and S. pogona-Sp1418. S. pogona-Δsp1418 exhibited a slow growth trend, especially in the logarithmic phase, in which the final biomass values of S. pogona-Δsp1418 were lower compared to those of wild type and S. pogona-Sp1418. S. pogona-Sp1418 showed a slightly slower logarithmic growth and entered stationary phase at 96 h, which was 24 h later compared to the wild-type strain, and lasted up to 180 h, which was greatly extended compared to the wild-type strain. Glucose consumption by the knockout strain was the slowest and exhausted in 168 h, which may be related to its growth restriction. The rate of glucose consumption in the logarithmic phase of the overexpression strain was slower, lasting for 48 h more than the wild-type bacteria, and the result was consistent with the growth curve. Error bars indicated standard errors of results from n = 3 replicates

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