Strain or plasmid | Characteristics | Reference or source |
---|---|---|
Strain | Â | Â |
 E. coli |  |  |
 DH5α | General cloning host | Invitrogen |
 BW25113/pIJ790 | Host for λ-Red recombination | [47] |
 ET12567/pUZ8002 | Donor strain for intergeneric conjugation | [48] |
S. lincolnensis | Â | Â |
 SyBE2901 | Original strain for high lincomycin-producer, derived from ATCC25466 | [46] |
 SyBE2904 | SyBE2901 ∆lmbU | This study |
 SyBE2913 | SyBE2904 with pLCY010-UYX | This study |
 SyBE2915 | SyBE2901 with pLCY010-U | This study |
 SyBE2929 | SyBE2904 with pLCY010-U | This study |
 SyBE2930 | SyBE2904 with pHZ1358-U | This study |
 SyBE2931 | SyBE2904 with pANT1201-U | This study |
Plasmid | Â | Â |
 SuperCos1 | pUC ori, kanr, ampr | Novagen |
 pIJ773 | Contains the aac(3)IV and oriT fragment | [47] |
 pUWL201apr | pUWL201 derivative, ampr, tsrr, aprr, oriT, carrying ermE* promoter | [49] |
 pHZ1358 | Replicative, tsrr, oriT | [50] |
 pANT1201 | Replicative, neor, oriT, carrying snpA promoter | [51] |
 pLCY010 | pUWL201 derivative, replicative, ampr, tsrr, hygr, carrying ermE* promoter | [46] |
 pAP07 | SuperCos1 derivative for deletion of lmbU, kanr, ampr, aprr | This study |
 pHZ1358-U | pHZ1358 with lmbU | This study |
 pANT1201-U | pANT1201 with lmbU | This study |
 pLCY010-U | pLCY010 with lmbU | This study |
 pLCY010-UYX | pLCY010 with lmbUYX | This study |