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Table 1 Strains and plasmids used in this study

From: Comparative transcriptomic analysis reveals the significant pleiotropic regulatory effects of LmbU on lincomycin biosynthesis

Strain or plasmid

Characteristics

Reference or source

Strain

  

 E. coli

  

 DH5α

General cloning host

Invitrogen

 BW25113/pIJ790

Host for λ-Red recombination

[47]

 ET12567/pUZ8002

Donor strain for intergeneric conjugation

[48]

S. lincolnensis

  

 SyBE2901

Original strain for high lincomycin-producer, derived from ATCC25466

[46]

 SyBE2904

SyBE2901 ∆lmbU

This study

 SyBE2913

SyBE2904 with pLCY010-UYX

This study

 SyBE2915

SyBE2901 with pLCY010-U

This study

 SyBE2929

SyBE2904 with pLCY010-U

This study

 SyBE2930

SyBE2904 with pHZ1358-U

This study

 SyBE2931

SyBE2904 with pANT1201-U

This study

Plasmid

  

 SuperCos1

pUC ori, kanr, ampr

Novagen

 pIJ773

Contains the aac(3)IV and oriT fragment

[47]

 pUWL201apr

pUWL201 derivative, ampr, tsrr, aprr, oriT, carrying ermE* promoter

[49]

 pHZ1358

Replicative, tsrr, oriT

[50]

 pANT1201

Replicative, neor, oriT, carrying snpA promoter

[51]

 pLCY010

pUWL201 derivative, replicative, ampr, tsrr, hygr, carrying ermE* promoter

[46]

 pAP07

SuperCos1 derivative for deletion of lmbU, kanr, ampr, aprr

This study

 pHZ1358-U

pHZ1358 with lmbU

This study

 pANT1201-U

pANT1201 with lmbU

This study

 pLCY010-U

pLCY010 with lmbU

This study

 pLCY010-UYX

pLCY010 with lmbUYX

This study