Fig. 5

Identification of the transcription units of the lmb cluster. a Visualization of the predicted TTSs distribution in the lmb gene cluster and FPKM of each genes by ssRNA-Seq. The predicted TTSs are shown by bent arrows. Pointed boxes indicate the transcription directions of genes. The FPKM value columns of genes in sense strand are represented by blue, and those in antisense are represented by orange. b Detection of upstream transcripts by RT-PCR. The 1st cDNAs were prepared using antisense primers of the downstream genes, and the ds-cDNAs were amplified using each pair of primers (arrowheads) of the upstream genes. The analysis was carried out three times for each reaction, and the identity of each amplification product was authenticated by direct sequencing. c Transcription units of the lmb cluster. The vertical arrows connect the adjacent lmb genes and point to the direction of the gene transcriptions