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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Comparative transcriptomic analysis reveals the significant pleiotropic regulatory effects of LmbU on lincomycin biosynthesis

Fig. 3

Deletion of the lmbU gene. a Schematic representation of the in-frame deletion of lmbU. A 617-bp region of lmbU was replaced by the 1370-bp aac(3)IV-oriT fragment through double crossover. b Confirmation of the lmbU deletion strain SyBE2904 by PCR amplification. Lane 1: a 1370-bp product using the original SyBE2901 genomic DNA as a template; Lane 2: a 2123-bp product using the SyBE2904 genomic DNA as a template; M: DNA molecular weight standard

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