Table 3 Bacterial strains and plasmids used in this study

E. coli strains

 DH5α

supE44, ∆lacU169(ϕ80 lacZ∆M15) hsdR17 recA1 endA1 hsdR gyrA relA thi

[53]

C. glutamicum strains

 ATCC13032

Wild-type

[54]

 ATCC13032 K9

htrA::htrA′-eypf (replacement of htrA by htrA′-eyfp)

This study

C. glutamicum plasmids

 pSenLys

Lysine sensor plasmid encoding LysG and its target promoter PlysE fused to eyfp

[44]

 pK19mobsacB

Vector for allelic exchange in C. glutamicum, pK18 oriV_E.coli, oriT, mob, sacB, lacZα, Km^R

[45]

 pK19mobsacB-K9

pK19mobsacB containing the eyfp gene flanked by the upstream region and the first 51 bp of the htrA structural gene and the htrA downstream region

This study

 pEKEx2

C. glutamicum/E. coli shuttle vector for regulated gene expression, pBL1 oriV_C.glutamicum, pUC18 oriV_{E. coli}, P_tac, lacI^q, Km^R

[55]

 pEKEx2-AmyE

pEKEx2 containing a gene encoding B. subtilis AmyE with its authentic signal peptide and a carboxyl-terminal His-tag

This study

 pEKEx2-ΔSP-AmyE

pEKEx2 containing a gene encoding a signal peptide-less variant of B. subtilis AmyE with a carboxyl-terminal His-tag

This study

 pEKEx2-NprE-cutinase

pEKEx2 containing a gene encoding the mature part of the cutinase from F. solani pisi fused to the NprE signal peptide

[10]

 pEKEx2-YpjP-cutinase

pEKEx2 containing a gene encoding the mature part of the cutinase from F. solani pisi fused to the YpjP signal peptide

[10]

 pEKEx2-YwmC-cutinase

pEKEx2 containing a gene encoding the mature part of the cutinase from F. solani pisi fused to the YwmC signal peptide

[10]