Fig. 1
From: A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum
The htrA locus of C. glutamicum. a Genomic organization of the chromosomal cgtR2-cgtS2-htrA-moa-cg1000 locus in C. glutamicum ATCC13032 wild-type. The promoters (P) upstream and within the operon and the transcripts derived from the respective promoters [25] are indicated. b In C. glutamicum K9, the htrA gene was replaced by a DNA fragment encompassing the first 51 bp of htrA (htrA′) followed by a TAG stop codon (underlined), a 16 bp spacer containing a ribosome binding site (RBS, bold letters in the nucleotide sequence) and the eyfp gene