Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in l-ornithine production

Table 2 Engineering the l-ornithine production in recombinant C. glutamicum

Strains	Cell biomass (OD₆₀₀)	l-ornithine accumulation (g/L)	l-ornithine/cell biomass (OD₆₀₀)
orn8	14.45 ± 0.35	19.58 ± 0.42	1.36
orn17	15.35 ± 0.15	17.66 ± 0.36	1.15
orn18	13.95 ± 0.10	20.28 ± 0.53	1.45
orn19	14.78 ± 0.18	17.81 ± 0.68	1.21
orn20	14.23 ± 0.53	22.01 ± 0.40	1.55
orn21	14.40 ± 0.05	20.79 ± 0.11	1.44
orn22	15.85 ± 0.75	22.52 ± 0.49	1.42
orn23	15.08 ± 0.73	21.86 ± 0.08	1.45
orn24	15.45 ± 0.05	21.84 ± 0.91	1.41
orn25	14.20 ± 0.35	18.87 ± 0.55	1.33
orn26	14.33 ± 0.28	17.18 ± 0.17	1.20
orn27	14.35 ± 0.60	16.93 ± 0.42	1.18
orn28	14.88 ± 0.03	23.83 ± 0.19	1.60
orn29	14.58 ± 0.13	25.46 ± 0.23	1.75
Orn30	15.43 ± 0.28	25.88 ± 0.79	1.68

Fermentations were performed at 250 rpm for 72 h, and the initial glucose concentration was 100 g/L
Results are the means ± standard deviations in three individual experiments

ISSN: 1475-2859