Table 1 Bacterial strains and plasmids used in this study
Designation | Genotype or description | Reference |
|---|---|---|
L. lactis strains | ||
MG1363 | Plasmid free L. lactis strain | [30] |
FS1072 | MG1363 △3ldh △pta △adhE △als | (Suo et al.) |
FS1080 | FS1072 containing the pLP712 plasmid | (Suo et al.) |
ll-cat01 | MG1363 ΔldhB ΔldhX Δpta ΔadhE ΔbutBA ΔaldB | This work |
RD03 | pLC0 in ll-cat01 | This work |
RD04 | pLC17 in ll-cat01 | This work |
RD05 | pLC02 in ll-cat01 | This work |
RD06 | pLC01 in ll-cat01 | This work |
Plasmids | ||
pLP712 | Plasmid from L. lactis NCDO712 encoding lactose utilization gene custer | [30] |
pLC0 | pTD6 derived plasmid | This work |
pLC01 | pLC0 inserted with the als gene from Enterococcus faecalis ATCC 29212 (ef-ALS) | This work |
pLC02 | pLC0 inserted with the als gene from Bacillus licheniformis WX-02 (bl-ALS) | This work |
pLC17 | pLC0 inserted with the als gene from Lactococcus lactis MG1363 (ll-ALS) | This work |