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Table 3 Mature serratiopeptidase specific PCR primers used for gene amplification

From: Enhanced production of recombinant serratiopeptidase in Escherichia coli and its characterization as a potential biosimilar to native biotherapeutic counterpart

Oligonucleotides

Sequence

Restriction site

Msrp_fwd_7298

5′-TATAATACTCATATGGCCGCGACAACC-3′

NdeI

Msrp_rev_7298

5′-ATGTACCTCGAGTTACACGATAAAGTCC-3′

XhoI

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Microbial Cell Factories

ISSN: 1475-2859

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