Table 2 Protein yield comparison at different steps of purification of the recombinant version of mature serratiopeptidase
Step | Yield (mg) | Purity (%) | Activity (EU/mg) |
|---|---|---|---|
Isolation | 130 ± 5 | 55 | Not measured |
Washing | 110 ± 10 | 60–65 | Not measured |
Refolding by dialysis | 57.5 ± 2.5 | 85–90 | 1650 ± 5 EU/mg |
Gel filtration | 47.5 ± 2.5 | > 98 | 1750 ± 5 EU/mg |