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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhanced production of recombinant serratiopeptidase in Escherichia coli and its characterization as a potential biosimilar to native biotherapeutic counterpart

Fig. 3

Optimization of serratiopeptidase expression in E. coli C43(DE3) cells. a Effect of induction on overexpression of mature serratiopeptidase in E. coli C43(DE3) cells at different time points of growth was observed by supplementing culture with 1 mM IPTG. Section 1 shows the SDS-PAGE gel loaded with induced samples at different time points. Section 2 shows the bar graph plot representing the relative expression percentage of mature serratiopeptidase as the average of three independent densitometric analysis. Section 1 of part b shows the overexpression profile of the recombinant version of mature serratiopeptidase in different growth media on 12% SDS-PAGE gel. Obtained percent values of serratiopeptidase expression in terms of total intracellular protein expression was averaged out for three independent densitometric analysis and used for plotting the bar graph shown in Section-2 of part-b

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