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Table 1 Distribution of protonation states of itaconate in controlled high-density pulsed fed-batch fermentation of U. cynodontis ∆fuz7 at different pH values

From: Process engineering of pH tolerant Ustilago cynodontis for efficient itaconic acid production

pH

H2ITA (g L−1)

HITA− (g L−1)

ITA2− (g L−1)

Total titer (g L−1)

1.9

0.0 ± 0.0

0.0 ± 0.0

0.0 ± 0.0

0.0 ± 0.0

2.5

14.1 ± 1.2

0.9 ± 0.1

0.0 ± 0.0

15.0 ± 1.2

3.2

15.9 ± 0.4

5.0 ± 0.1

0.1 ± 0.0

20.9 ± 0.5

3.4

14.6 ± 0.7

7.2 ± 0.3

0.1 ± 0.0

22.0 ± 1.1

3.6

13.7

10.7

0.3

24.7

3.8

10.7 ± 0.2

13.3 ± 0.2

0.6 ± 0.0

24.6 ± 0.4

5.5

0.1 ± 0.0

6.2 ± 0.1

13.8 ± 0.2

20.1 ± 0.4

6

0.0 ± 0.0

2.1 ± 0.0

14.9 ± 0.0

17.0 ± 0.0

  1. Errors indicate the deviation from the mean (n = 2) with the exception of the fermentation at pH 3.6, which shows a single representative culture