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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Process engineering of pH tolerant Ustilago cynodontis for efficient itaconic acid production

Fig. 6

Repeated batch with cell recycling of U. cynodontis NBRC 9727 ∆fuz7r ∆cyp3r PetefmttA Pria1ria1. a OD600 (▲) and pH (green line); b glucose (■) itaconate () and total itaconate (, dashed line) concentration, and used NaOH (red line) during a single representative fermentation in a bioreactor containing batch medium with 200 g L−1 glucose, 4 g L−1 NH4Cl at pH 3.6 titrated with 10 M NaOH (a, b), or in shake flasks containing screening medium with 33 g L−1 CaCO3 and 50 g L−1 glucose (c). For the repeated batches (indicated by grey shading), culture broth was centrifuged and the biomass was subsequently re-suspended in 0.5 L fresh batch medium without nitrogen, 170 g L−1 glucose and 0.5 g L yeast extract (a, b), or culture broth was centrifuged and the biomass was subsequently re-suspended in screening medium without nitrogen, 50 g L−1 glucose and 25 g L−1 CaCO3 (c). Error bars indicate the standard error from the mean (n = 4)

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