Improved utilization of soybean meal through fermentation with commensal Shewanella sp. MR-7 in turbot (Scophthalmus maximus L.)

Li, Chaoqun; Zhang, Beili; Wang, Xin; Pi, Xionge; Wang, Xuan; Zhou, Huihui; Mai, Kangsen; He, Gen

doi:10.1186/s12934-019-1265-z

Table 6 Formulas and proximate composition of the experimental diets (% dry matter)

From: Improved utilization of soybean meal through fermentation with commensal Shewanella sp. MR-7 in turbot (Scophthalmus maximus L.)

Ingredients	Treatments
Ingredients	FM	SBM15	SBM30	SBM45	SBM60	FSBM15	FSBM30	FSBM45	FSBM60
Fish meal^a	60.00	51.00	42.00	33.00	24.00	51.00	42.00	33.00	24.00
Soybean meal^a	–	12.34	24.69	37.03	49.37	–	–	–	–
Fermented soybean meal^b	–	–	–	–	–	11.95	23.90	35.86	47.81
Wheat meal^a	24.94	18.00	13.46	8.30	3.48	18.49	14.06	9.77	4.81
Wheat gluten meal^a	2.46	3.73	4.51	5.30	6.27	3.63	4.39	5.00	6.21
Fish oil	4.60	4.80	5.10	5.90	6.40	4.80	5.40	5.90	6.70
Amino acid	0.00	0.12	0.25	0.47	0.48	0.12	0.25	0.47	0.47
Taurine	0.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
Vitamin premix^c	2.00	2.00	2.00	2.00	2.00	2.00	2.00	2.00	2.00
Mineral premix^d	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
Attractant^e	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
Cholesterol	0.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00	1.00
Others^f	4.00	4.00	4.00	4.00	4.00	4.00	4.00	4.00	4.00
Analytical composition (dry matter, %)
Energy (kJ g⁻¹)	20.19	20.24	20.27	20.38	20.49	20.15	20.15	20.11	20.20
Crude protein	50.00	50.00	50.00	50.00	50.00	50.00	50.00	50.00	50.17
Crude lipid	12.19	12.79	12.49	12.69	12.60	12.73	12.67	12.51	12.65

FM diet fish meal, SBM15 replacement of 15% fish meal protein by soybean meal protein, SBM30 replacement of 30% fish meal protein by soybean meal protein, SBM45 replacement of 45% fish meal protein by soybean meal protein, SBM60 replacement of 60% fish meal protein by soybean meal protein, FSBM15 replacement of 15% fish meal protein by fermented soybean meal protein, FSBM30 replacement of 30% fish meal protein by fermented soybean meal protein, FSBM45 replacement of 45% fish meal protein by fermented soybean meal protein, FSBM60 replacement of 60% fish meal protein by fermented soybean meal protein
^aSupplied by Great seven Bio-Tech (Qingdao, China); fish meal, crude protein, 73.77%, crude lipid, 7.58%; soybean meal, crude protein, 52.97%, crude lipid, 2.12%; wheat meal, crude protein, 17.82%, crude lipid, 2.24%; wheat gluten meal, crude protein, 83.31%, crude lipid, 1.75%
^bFermented soybean meal got from soybean meal fermented with Shewanella sp. MR-7, crude protein, 54.70%, crude lipid, 1.66%
^cVitamin premix (mg kg⁻¹ diet): retinal palmitate, 32; cholecalciferol, 5; DL-α-tocopherol acetate, 240; menadione, 10; thiamin-HCl, 25; riboflavin, 45; pyridoxine–HCl, 20; cyanocobalamin, 10; D-calcium pantothenate, 60; amine nicotinic acid, 200; folic acid, 20; biotin, 60; mesoinositol, 800; ascorbyl polyphosphate (contained 35% ascorbic acid), 2000; microcrystalline cellulose, 16,473
^dMineral premix (mg kg⁻¹ diet): MgSO₄·7H₂O, 1200; CuSO₄·5H₂O, 10; FeSO₄·H₂O, 80; ZnSO₄·H₂O, 50; MnSO₄·H₂O, 45; CoCl₂·6H₂O (1%), 50; Na₂SeO₃ (1%), 20; calcium iodine, 60; zeolite, 8485
^eAttractant:betaine:dimethyl-propiothetin:glycine:alanine:5-phosphateinosine = 4:2:2:1:1
^fOthers (10 g kg⁻¹ diet): soy lectithin, 2.00; monocalcium phosphate, 1.00; choline chloride, 0.30; Yttrium oxide, 0.10; calcium propionic acid, 0.05; ethoxyquin, 0.05; sodium alginate, 0.50

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ISSN: 1475-2859

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