Table 1 Summary of all gene and genome editing reports in Y. lipolytica

Cas9 indel and integration

Hybrid promoters combining strong RNA pol II promoters and tRNA lead to high efficiency indel

70007

[51]

Cas9 indels, multiplexed

Multiplexed indels by multiple sgRNAs processed with hammerhead ribozymes

73226

[52]

Cas9 indels

T7 polymerase expression of sgRNA; Addition of GGG improved sgRNA activity

N/A

[53]

Cas9 integration

Non-essential landing sites were established for markerless integration

84608-17

[27]

Cas9 deletion and integration

Two sgRNAs used to excise DNA regions. Simultaneous integration by HMEJ

N/A

[55]

Cas9 integration

EasyCloneYALI: integration of non-replicating constructs by HR at 11 landing sites in Ku70 knockout

100000 0140-141

[56]

TALENs indel and integration

TALEN induced DSB can create indels or mediate HR based integration

N/A

[57]

dCas9-MxiI

sgRNA targeted upstream of the TSS

91248

[58]

dCas9

dCpfI

Multiplexed targeting of both dCas9 and dCpfI a pathway

N/A

[59]

CRISPR-VPR

CRISPRa upregulated two genes for cellobiose metabolism

N/A

[60]

PiggyBac

TTAA specific transposon insertion; excision/integration mutant used for recycle selection markers

N/A

[66]

Hermes Tn

Sequence independent transposon insertion; useful for functional genomics and strain engineering

113332

[67]

Cas9 genome scale indel

Sixfold redundant sgRNA for entire genome; useful for functional genomics and strain engineering

N/A

[68]