Fig. 1From: Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolyticaCRISPR–Cas9 innovations. a Hybrid promoters of RNA polymerase III promoters and tRNA lead to efficient sgRNA expression and processing. b Inclusion of gRNA target sites on the transgene plasmid lead to two possible site specific modes of integration—homologous recombination (HR) and HR-independent homology mediated end joining (HMEJ)Back to article page