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Table 2 Plasmids constructed for genomic integrations

From: Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid

NamePlasmid backboneaIntegrated expression cassettesIntegration site (gRNA vector used)Verification primersa
pCfB2904-ABA1pCfB2904PPGK1-bcaba1-TADH1XI-3 (pCfB3052)2221/911
pCfB2904-ABA1-CPRpCfB2904PPGK1-bcaba1-TADH1 PTEF1-bccpr1-TCYC1XI-3 (pCfB3052)2221/911
pCfB2904-ABA1-NCP1pCfB2904PPGK1-bcaba1-TADH1 PTEF1-NCP1-TCYC1XI-3 (pCfB3052)2221/911
pCfB2909-ABA2-ABA4pCfB2909PPGK1-bcaba2-TADH1 PTEF1-bcaba4-TCYC1XII-5 (pCfB3052)2221/899
pCfB3036-bcceP450pCfB3036PPGK1-bcceP450-TADH1XI-1 (pCfB3043)2221/907
pCfB3035-ABA3-ABA5pCfB3035PPGK1-bcaba5-TADH1 PTEF1-bcaba3-TCYC1X-4 (pCfB3052)2221/905
pCfB3035-ABA3pCfB3035PTEF1-bcaba3-TCYC1X-4 (pCfB3052)2221/905
pCfB3035-ABA5pCfB3035PPGK1-bcaba5-TADH1X-4 (pCfB3052)2221/905
  1. aSee Easyclone-MarkerFree manual [37] for detailed sequence information