Table 2 Plasmids constructed for genomic integrations
Name | Plasmid backbonea | Integrated expression cassettes | Integration site (gRNA vector used) | Verification primersa |
|---|---|---|---|---|
pCfB2904-ABA1 | pCfB2904 | PPGK1-bcaba1-TADH1 | XI-3 (pCfB3052) | 2221/911 |
pCfB2904-ABA1-CPR | pCfB2904 | PPGK1-bcaba1-TADH1 PTEF1-bccpr1-TCYC1 | XI-3 (pCfB3052) | 2221/911 |
pCfB2904-ABA1-NCP1 | pCfB2904 | PPGK1-bcaba1-TADH1 PTEF1-NCP1-TCYC1 | XI-3 (pCfB3052) | 2221/911 |
pCfB2909-ABA2-ABA4 | pCfB2909 | PPGK1-bcaba2-TADH1 PTEF1-bcaba4-TCYC1 | XII-5 (pCfB3052) | 2221/899 |
pCfB3036-bcceP450 | pCfB3036 | PPGK1-bcceP450-TADH1 | XI-1 (pCfB3043) | 2221/907 |
pCfB3035-ABA3-ABA5 | pCfB3035 | PPGK1-bcaba5-TADH1 PTEF1-bcaba3-TCYC1 | X-4 (pCfB3052) | 2221/905 |
pCfB3035-ABA3 | pCfB3035 | PTEF1-bcaba3-TCYC1 | X-4 (pCfB3052) | 2221/905 |
pCfB3035-ABA5 | pCfB3035 | PPGK1-bcaba5-TADH1 | X-4 (pCfB3052) | 2221/905 |