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Table 1 S. cerevisiae strains used in this study

From: Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid

 Strain nameGenotypeReferences
Background strainsCEN.PK113-5DMATa MAL2-8 c SUC2 ura3-52[76], provided by P. Kötter, University of Frankfurt, Germany
5D-tHMG1MATa MAL2-8 c SUC2 ura3-52 PTEF1 -tHMG1This study
SCIGS22aMATa MAL2-8 c SUC2 ura3-52 lpp1Δ::loxP dpp1Δ::loxP PERG9 Δ::loxP-PHXT1 gdh1Δ::loxP PTEF1 -ERG20 PPGK1 -GDH2 PTEF1 -tHMG1[43]
Strains with B. cinerea genesDABA1MATa MAL2-8 c SUC2 ura3-52
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4 PPGK1-bcaba5 PTEF1-bccpr1 PPGK1-bcceP450
This study
TABA1MATa MAL2-8c SUC2 ura3-52 PTEF1 -tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4 PPGK1-bcaba5 PTEF1-bccpr1 PPGK1-bcceP450
This study
SABA1MATa MAL2-8 c SUC2 ura3-52 lpp1Δ::loxP dpp1Δ::loxP PERG9 Δ::loxP-PHXT1 gdh1Δ::loxP P TEF1 -ERG20 PPGK1 -GDH2 PTEF1-tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4 PPGK1-bcaba5 PTEF1-bccpr1 PPGK1-bcceP450
This study
TABA2MATa MAL2-8c SUC2 ura3-52 PTEF1 -tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4 PPGK1-bcaba5 PTEF1-bccpr1
This study
TABA3MATa MAL2-8 c SUC2 ura3-52 PTEF1 -tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4 PTEF1-bccpr1
This study
SABA3MATa MAL2-8 c SUC2 ura3-52 lpp1Δ::loxP dpp1Δ::loxP PERG9 Δ::loxP-PHXT1 gdh1Δ::loxP P TEF1 -ERG20 PPGK1 -GDH2 PTEF1-tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4 PTEF1-bccpr1
This study
TABA4MATa MAL2-8 c SUC2ura3-52 PTEF1 -tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba4 PPGK1-bcaba5 PTEF1-bccpr1
This study
TABA5MATa MAL2-8 c SUC2 ura3-52 PTEF1 -tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4 PTEF1-NCP1
This study
TABA6MATa MAL2-8 c SUC2 ura3-52 PTEF1 -tHMG1
PPGK1-bcaba1 PPGK1-bcaba2 PTEF1-bcaba3 PTEF1-bcaba4
This study
S3S0SABA3 with p416 emptyThis study
S3S1SABA3 with p416-ABA1This study
S3S2SABA3 with p416-ABA2This study
S3S3SABA3 with p416-ABA3This study
S3S4SABA3 with p416-ABA4This study
S3S12SABA3 with p416-ABA1 + 2This study