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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid

Fig. 2

HPLC–MS analysis of ABA-producing strains and controls. a Chromatograms of extracted supernatants after 48 h of cultivation of the strains 5D-tHMG1, 5D-tHMG1 spiked with ABA standard, TABA1 (5D-tHMG1 carrying bcaba12345, bccpr1 and bcceP450), and (S)-(+)-ABA standard dissolved in methanol. One replicate is displayed per strain. Retention time is displayed on top of the peaks. The chromatograms were smoothed and a blank methanol run was subtracted to remove impurities. The strains DABA1 and SABA1 showed the same chromatogram peaks as TABA1 (Figure S1 in Additional file 1). Chromatograms of all strains without the subtraction of the methanol blank run can be found in Figure S1 in Additional file 1. RT retention time. b ABA titre in supernatant and OD600 of strains containing bcaba12345, bccpr1 and bcceP450. Strains were cultivated for 48 h in minimal medium supplemented with uracil. DABA1 is based on the genetic background of 5D, TABA1 is based on 5D-tHMG1 and SABA1 is based on SCIGS22a. Average ABA titres and maximum OD600 were calculated from three independent biological replicates

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