Synthetic control devices for gene regulation in Penicillium chrysogenum

Table 2 Penicillin V production in shake flask cultures and characteristics of strains where the penicillin cluster was put under the control of the synthetic transcriptional factor

Strain no.	Promoter for expression of pcbAB	QUAS elements of pcbAB-pcbC locus	QUAS elements of penDE locus	Penicillin V titer (g/L)^a
Strain no.	Promoter for expression of pcbAB	QUAS elements of pcbAB-pcbC locus	QUAS elements of penDE locus	After 3 days	After 5 days	After 7 days
DS54468	Pc_pcbAB	–	–	0.34 ± 0.016	0.48 ± 0.023	0.48 ± 0.006
23	Pc_pcbAB CP	5xQUAS	5xQUAS	0.04 ± 0.001	0.06 ± 0.000	0.07 ± 0.002
24	Pc_pcbAB CP	11xQUAS	5xQUAS	0.05 ± 0.002	0.08 ± 0.001	0.14 ± 0.005
25	Pc_pcbAB full	5xQUAS	5xQUAS	0.14 ± 0.002	0.26 ± 0.002	0.33 ± 0.011
26	Pc_pcbAB full	11xQUAS	5xQUAS	0.20 ± 0.003	0.38 ± 0.003	0.49 ± 0.006

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