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Table 1 Strains characterized by fluorescence measurements

From: Synthetic control devices for gene regulation in Penicillium chrysogenum

No.

Description of strain

Number of QUAS elements (#2)

Promoter for expression of RFP (#3)

Gene associated with promoter for expression of RFP

1

40s_1xQ_pcbC

1

Pc_pcbC CP

Pc21g21380

2

40s_5xQ_pcbC

5

Pc_pcbC CP

Pc21g21380

3

40s_11xQ_pcbC

11

Pc_pcbC CP

Pc21g21380

4

40s_pcbC_CP

Pc_pcbC CP

Pc21g21380

5

gndA_pcbC_CP

Pc_pcbC CP

Pc21g21380

6

40s_5xQUAS

5

7

40s_pcbAB_CP

Pc_pcbAB CP

Pc21g21390

8

40s_penDE_CP

Pc_penDE CP

Pc21g21370

9

40s_phl_CP

Pc_phl CP

Pc22g14900

10

40s_nirA_CP

An_nirA CP

AN0098

11

40s_ura3_CP

Sc_ura3 CP

YEL021 W

12

40s_5xQ_pcbAB

5

Pc_pcbAB CP

Pc21g21390

13

40s_5xQ_penDE

5

Pc_penDE CP

Pc21g21370

14

40s_5xQ_phl

5

Pc_phl CP

Pc22g14900

15

40s_5xQ_nirA

5

An_nirA CP

AN0098

16

40s_5xQ_ura3

5

Sc_ura3 CP

YEL021 W

17

40s_5xQ_reverse_pcbC

5

Pc_pcbC CP

Pc21g21380

18

gndA_5xQ_pcbC

5

Pc_pcbC CP

Pc21g21380

19

gndA_5xQ_nirA

5

An_nirA CP

AN0098

20

gndA_5xQ_ura3

5

Sc_ura3 CP

YEL021 W

21

40s_pcbC full

Pc_pPcbC

Pc21g21380

22

40s_pcbAB full

Pc_pPcbAB

Pc21g21390

  1. Elements varied are marked with numbers in Fig. 1. The STF transcribed under the p40S (An11g02040) promoter (#1) or the pgndA (AN0465) promoter (strains 5, 18–20). All strains were derived from DS68530